Tax Levy Financing for Local Public Health: Relationships between Fiscal Allocation, Fiscal Effort and Fiscal Capacity

This study examines property tax levy (local tax levy) as a source of local health department (LHD) funding during a five year period (2006-2010) in all Minnesota counties by assessing fiscal effort, fiscal allocation and fiscal capacity. Local health departments rely on pluralistic funding from local, state, federal and private sources. However, local tax levy funding is unexplored and little is known regarding the extent of fiscal allocation (tax levy used for LHD), fiscal effort (potential amount of tax levy available for LHD), and fiscal capacity (wealth of community). More important it is not known to what extent variation between local jurisdictions fluctuated over time, how they are offset by declining funding from other sources, or whether other sources supplement total tax levy reductions. It is essential to explore these issues to provide a basic understanding of fiscal drivers for ongoing services. Our findings indicate that from 2006 to 2010 the local tax levy for public health as a percent of total local health department expenditures decreased 6.7%, while local tax levy for public health as a percent of total tax levy decreased 14.6%. However, during this time period the total per capita tax levy for all services increased 25.2%

Intracavity Raman conversion of a red semiconductor disk laser using diamond

We demonstrate a diamond Raman laser intracavity-pumped by a red semiconductor disk laser (~675 nm) for laser emission at around 740 nm. Output power up to 82 mW of the Stokes-shifted field was achieved, limited by the available pump power, with an output coupling of 1.5%. We also report wavelength tuning of the diamond Raman laser over 736 - 750 nm

~1400-nm continuous-wave diamond Raman laser intracavity-pumped by an InGaAs semiconductor disk laser

We present a ~1400nm-emitting diamond Raman laser intracavity-pumped by an ~1180nm semiconductor disk laser. We measured a maximum output power of 2.3 W at ~1400nm with an output coupling of 3.5%. The Raman laser was tunable from 1373 to 1415nm using a 4-mm-thick birefringent filter

Continuous-wave Raman laser pumped within a semiconductor disk laser cavity

A KGd(WO4)(2) Raman laser was pumped within the cavity of a cw diode-pumped InGaAs semiconductor disk laser (SDL). The Raman laser threshold was reached for 5: 6W of absorbed diode pump power, and output power up to 0.8W at 1143nm, with optical conversion efficiency of 7.5% with respect to the absorbed diode pump power, was demonstrated. Tuning the SDL resulted in tuning of the Raman laser output between 1133 and 1157nm

InGaAs-QW VECSEL emitting >1300nm via intracavity Raman conversion

We report intracavity Raman conversion of a long-wavelength InGaAs-QW VECSEL to ~1320 nm, the longest wavelength yet achieved by a VECSEL-pumped Raman laser. The setup consisted of a VECSEL capable of emitting >17W at 1180nm and tunable from 1141-1203nm and a 30-mm-long KGd(WO4)2 (KGW) Raman crystal in a coupled-cavity Raman resonator. The Raman cavity was separated from the VECSEL resonator by a tilted dichroic mirror, which steers the Raman beam to an output coupler external to the VECSEL. The spectral emission of the VECSEL, and consequently of the Raman laser, was set by a 4-mm-thick quartz birefringent filter in the VECSEL cavity. The KGW Raman laser was capable of emitting 2.5W at 1315 nm, with M2~2.7 and >4% diode-to-Stokes conversion efficiency. The Raman laser emission was tunable from 1295-1340 nm, limited by the free spectral range of the birefringent filter. Spectral broadening of the fundamental emission was observed during Raman conversion. At the maximum Raman laser output power, the total linewidth of the VECSEL spectrum was ~0:7nm FWHM. As a consequence, the Raman laser emission was also relatively broad (~0.9nm FWHM). Narrow (<0.2nm FWHM) Raman emission was obtained by inserting an additional 100 µm etalon within the VECSEL cavity. With this configuration the fundamental intracavity power clamped at its value at the Raman threshold, suggesting an enhanced effective Raman gain, but the maximum output power of the Raman laser was 1.8 W

A framework to evaluate land degradation and restoration responses for improved planning and decision-making.

Made available in DSpace on 2020-05-14T20:20:36Z (GMT). No. of bitstreams: 1 A-framework-to-evaluate-land-degradation-and-restoration-responses-for-improved-planning-and-decision-making.pdf: 3147852 bytes, checksum: e97d8b89271bd75dfcdff4bd682b112d (MD5) Previous issue date: 2020bitstream/item/212992/1/A-framework-to-evaluate-land-degradation-and-restoration-responses-for-improved-planning-and-decision-making.pd

Biochemical and cytological interactions between callose synthase and microtubules in the tobacco pollen tube

Key message: The article concerns the association between callose synthase and cytoskeleton by biochemical and ultrastructural analyses in the pollen tube. Results confirmed this association and immunogold labeling showed a colocalization. Abstract: Callose is a cell wall polysaccharide involved in fundamental biological processes, from plant development to the response to abiotic and biotic stress. To gain insight into the deposition pattern of callose, it is important to know how the enzyme callose synthase is regulated through the interaction with the vesicle-cytoskeletal system. Actin filaments likely determine the long-range distribution of callose synthase through transport vesicles but the spatial/biochemical relationships between callose synthase and microtubules are poorly understood, although experimental evidence supports the association between callose synthase and tubulin. In this manuscript, we further investigated the association between callose synthase and microtubules through biochemical and ultrastructural analyses in the pollen tube model system, where callose is an essential component of the cell wall. Results by native 2-D electrophoresis, isolation of callose synthase complex and far-western blot confirmed that callose synthase is associated with tubulin and can therefore interface with cortical microtubules. In contrast, actin and sucrose synthase were not permanently associated with callose synthase. Immunogold labeling showed colocalization between the enzyme and microtubules, occasionally mediated by vesicles. Overall, the data indicate that pollen tube callose synthase exerts its activity in cooperation with the microtubular cytoskeleton

Antisense-mediated exon skipping: a therapeutic strategy for titin-based dilated cardiomyopathy

Frameshift mutations in the TTN gene encoding titin are a major cause for inherited forms of dilated cardiomyopathy (DCM), a heart disease characterized by ventricular dilatation, systolic dysfunction, and progressive heart failure. To date, there are no specific treatment options for DCM patients but heart transplantation. Here, we show the beneficial potential of reframing titin transcripts by antisense oligonucleotide (AON)-mediated exon skipping in human and murine models of DCM carrying a previously identified autosomal-dominant frameshift mutation in titin exon 326. Correction of TTN reading frame in patient-specific cardiomyocytes derived from induced pluripotent stem cells rescued defective myofibril assembly and stability and normalized the sarcomeric protein expression. AON treatment in Ttn knock-in mice improved sarcomere formation and contractile performance in homozygous embryos and prevented the development of the DCM phenotype in heterozygous animals. These results demonstrate that disruption of the titin reading frame due to a truncating DCM mutation canbe restored by exon skipping in both patient cardiomyocytes invitro and mouse heart invivo, indicating RNA-based strategies as a potential treatment option for DCM